Tens of thousands of people again marched through the centre of The Hague on Sunday to protest against the Dutch government’s position on Israel and the war in Gaza.

The protest organisers estimated attendance at more than 150,000 people, making it larger then the first Red Line protest held in May.

Many of the demonstrators wore red clothing to symbolically draw a “red line”, which they say is something the government had previously refused to do.

The protest was put together by a broad coalition of human rights and pro-Palestinian groups, including Amnesty International, Médecins Sans Frontières, Save the Children, Pax, Oxfam Novib, The Rights Forum and Plant een Olijfboom.

“The Dutch cabinet still refuses to draw a red line. That is why we do it, for as long as necessary,” Marjon Rozema of Amnesty International Netherlands said in a statement.

The organisers called on the Dutch government to take concrete action to stop Israel’s continued violations of international humanitarian law.

The crowd was mixed and, according to the police in The Hague, the protest was peaceful.

In May, the European Union announced it will review its political and economic agreement with Israel because of the “catastrophic” situation in Gaza, according the bloc’s top diplomat Kaja Kallas.

Dutch foreign minister Caspar Veldkamp led the initiative in a letter to Kallas calling for a review of the EU-Israel Association Agreement in what has been seen domestically as strongest criticism of Israel by the Dutch government since the bombardment of Gaza began.

Earlier this week, caretaker prime minister Dick Schoof said he was not ruling out the Netherlands asking for EU sanctions to be imposed on two far-right Israeli ministers for incitement to extremist violence and violation of Palestinian human rights.

Other red line protests were held this weekend as well and more have been planned for later in the month.

On the island of Terschelling, around 2,000 people dressed in red formed a red line on the beach during the Oerol theatre festival, according to broadcaster NOS. According to the organisers, it was a way for visitors, artists and islanders to express solidarity with the protest in The Hague.

Schoof issued a statement later on Sunday in which he referred to the “thousands of people” raising their voices for Gaza “expressing concern, anger and frustration”.

The Netherlands, he said, remains committed to ending the violence and lifting the humanitarian blockade. “We continue to assess how we can be most effective, both publicly and behind the scenes, in improving the situation on the ground,” he said.

“To all those gathered in The Hague, I say: we see you and we hear you. Our ultimate goal is the same — to end the suffering in Gaza as soon as possible.”

Schoof and other ministers were criticised after the first Red Line demonstration in the city for failing to comment on the gathering.